I had missed this (or, rather, Google Alerts had). AlphaVax had apparently given a presentation titled Flow-Cytometric Evaluation of T-Cell Responses Elicited by an Alphavirus Replicon Particle Vaccine for Cytomegalovirus (CMV) in Healthy Adults sometime in June.

Quite a mouthful. The presentation describes how AlphaVax used multiparameter flow cytometry to explore one of their vaccine candidates. They then used – among other applications – SPICE 5 to do their data mining, visualization, and production of presentation-quality graphs for their presentation.

Samples were fixed and stored at 4°C until acquisition on an LSRII flow cytometer. Flow data was analyzed with FlowJo version 8.7 (Tree Star, Ashland, OR). Further data analysis was performed using software (PESTLE and SPICE, version 5.0) provided by M. Roederer and J. Nozzi, NIAID, NIH.

It makes me proud to know my work is directly helping researchers find vaccines for “what ails ya.” Very gratifying.

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