SPICE 5.0 was featured in a poster session at the 2008 NIH Research Festival at the National Institutes of Health on October 15th.
SPICE 5.0: An Analysis and Discovery Software for Complex Flow Cytometry Data
J.Nozzi, Y. Mohamoud, Y. Huyen, M. Roederer
2008 NIH Research Festival, National Institutes of Health (Poster Session)
Abstract
Multicolor flow cytometry measures numerous analytes simultaneously in a single sample. Laboratories need sophisticated software to analyze the vast amounts of data generated from multicolor flow cytometry. We present a solution to this problem with the data mining software SPICE 5.0 (Simplified Presentation of Incredibly Complex Evaluations). SPICE analyzes polychromatic flow cytometry data produced by FLOWJO and organizes the normalized data graphically. SPICE enables users to discover potential correlations in their experimental data within complex data sets. SPICE was originally developed by Dr. Mario Roederer in the NIAID Vaccine Research Center (VRC) ImmunoTechnology Section. Under Dr. Roederer’s guidance, the NIAID Bioinformatics and Scientific IT Program (BSIP) completely redesigned the software to launch “SPICE 5.0”. SPICE 5.0 offers an enhanced user interface and adds flexibility for future expansion into additional input types. The SPICE 5.0 software is currently being used in the VRC ImmunoTechnology lab to reveal relationships among numerous T- cell immune responses resulting from vaccine challenges. Many potential applications for SPICE 5.0 exist: the software can be used to analyze any multivariate dataset for which a series of nominal measurements and a single continuous measurement is available.
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Spice Web Site
Spice is a product of NIH/NIAID and is hosted here: http://exon.niaid.nih.gov/spice
